Electron beam evaporation (AJA International Inc., MA, USA) formed a thin film of chromium (thickness, 10 nm) as an adhesion layer, followed by a layer of gold (thickness, 100 nm) as a conductor on a 75-μm-thick sheet of polyimide (Argon Inc., CA, USA). A UV laser (LPKF, Germany) patterned the gold-coated polyimide sheet to define the circular current collector, serpentine interconnects, and contact pads. The first step in realizing a biofuel cell–based lactate sensor involved punching out circular pads (diameter, 2 mm) of CNT paper (Buckypaper, GSM 20; NanoTechLabs, NC, USA). Coating with 2 μl of 0.1 M tetrathiafulvalene (Sigma-Aldrich, MO, USA) solution prepared in acetone/ethanol (1:9, v/v) and 4 μl of lactate oxidase (Toyobo Chemicals, Japan), and allowing them to dry, yielded enzyme-functionalized CNT pads. The enzyme solution resulted from dispersing the enzyme (60 mg/ml) in 0.1 M phosphate buffer containing 0.25 wt % glutaraldehyde (Sigma-Aldrich, MO, USA). Subsequently, drop-casting and drying 2 μl of chitosan [Chemical Abstracts Service (CAS) number 9012-76-4; Sigma-Aldrich, MO, USA] suspension prepared in 0.1 M acetic acid onto each pad formed a chitosan-based membrane. Dipping the dried pads into the chitosan solution for 5 s and then allowing them to dry resulted in an additional chitosan membrane. Last, dipping the pads for 5 s in 3 wt % PVC (CAS number 9002-86-2; Sigma-Aldrich, MO, USA) suspension in tetrahydrofuran and thoroughly air drying them formed the outer layer of PVC membrane. Conductive silver glue then bonded the pads to the gold current collectors to complete the anode functionalization process. The cathode for the lactate sensor resulted from drop-casting 15 μl of platinum black (10 mg/ml; Sigma-Aldrich, MO, USA) suspension prepared in deionized water, followed by applying 1 μl of Nafion 117 solution (Sigma-Aldrich, MO, USA), onto the cathode designated gold current collector. Storing the sensors at 4°C for at least 1 week before use allowed the chitosan and PVC membranes to stabilize. Fabrication of biofuel cell–based glucose sensors involved steps similar to those discussed for the lactate sensor with some modifications. The process began with drop-casting 1 μl of 0.1 M tetrathiafulvalene solution onto CNT pads. Separately, preparing a solution (40 mg/ml) of glucose oxidase in 0.1 M phosphate buffer containing bovine serum albumin (10 mg/ml; Sigma-Aldrich, MO, USA) and a 1 wt % suspension of Nafion in 0.1 M phosphate buffer and then mixing of the two suspensions in equal volumes yielded the enzyme-coating suspension. Application of 2 μl of the enzyme-coating suspension functionalized the tetrathiafulvalene-coated CNT pads. Conductive silver glue bonded the pads to the gold current collectors to complete the anode. The glucose sensor cathode resulted from preparing a suspension (10 mg/ml) of 10% platinum on carbon (Sigma-Aldrich, MO, USA) in a 2 wt % ethanolic suspension of Nafion, followed by casting 5 μl of the suspension on each current collector. Storing the sensors at 4°C for at least 1 week before use allowed the Nafion membrane to equilibrate. Both the lactate and glucose sensors were stable for at least 6 months when stored at 4°C without any additional storing conditions. Before use, attachment of the NFC module to the wearable patch and exposure of glucose sensor to buffer solution [0.1 M sodium phosphate buffer (pH 7)] for 1 hour resulted in stabilized signals for micromolar detection in sweat. Lactate sensor required no such conditioning step.
Instagrammers and science lovers take note—this JJ Young Pore Bubble Mask is basically a facial science experience you’ll want to document on camera. It’s one of the bestselling drugstore beauty products partially because it’s a great affordable face mask, but we’re pretty sure people are just falling in love with the oodles of bubbles it creates once it’s on your face. Imagine gentle pop rocks on your skin. Boom!
Dead mice, dirty food slicers, a dog in the dining area, and a fruit fly on a donut, also caught their attention. There were some notable names in this round of Clean Plates including a center city Dairy Queen, Dunkin Donuts, Shiroi Hana, Brigantessa and The Independent Hotel.
• The Bent Nail, 5217 98th St., Ste. 900: observed soiled kitchen utensils. Observed improper employee hand washing. Observed several expired items available for consumption. Observed improper storage of toxic items. Observed improper storage of bulk spice rub. Observed personal employee items stored in dish-sanitation area. Corrected on site.
Part of being a pro organizer is knowing when something should live outside your camper instead of inside. We love our gear garage in our van as it allows us to store all of the bikes and skis we need. But larger gear like stand up paddle boards need special racks, like these surf hooks that mount to a ladder and pole and swivel when not in use.
Luke, radishes are OK for your dog as a treat; some dogs may not like the spicy zip of a radish. Other veggies that are fine for the dog as a special snack: cauliflower, Brussels sprouts, broccoli, carrots, cucumbers, asparagus, green beans and potatoes, all either raw or cooked. They are a good source of vitamins. But no onions. And don't be confused: Wild radish is a wildflower that is poisonous. — Heloise
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Since I'm obviously not a professional athlete, I was just curious to find out my sweat rate running in the heat for a half hour. By taking an ingestible core body temperature sensor pill, which wirelessly transmitted readings to a recorder device outside my body, measuring my weight before and after the test, and exactly how much water I drank during the heated run, I was able to learn that I lose .74 liters per hour. That means that at that same temperature, if I were to run an hour, the perfect amount of fluid I should be taking in would be .74 liters because that's how much I was sweating out.
• Optimal growth. Bare root plants acclimate more readily to new soil conditions than do actively growing plants. Also, since they have no leaves or flowers to maintain, they can put all their energy into developing a strong root system after planting.
In a secure vault in the suburbs of Paris, an egg-sized cylinder of metal sits in a climate-controlled room under three glass bell jars. It is the mass against which all other masses in the world are measured - by definition ...
Whether you hit the same salad spot for lunch every day, or prefer to take your chances at your workplace canteen, the weekly waste of those paper bowls and plastic cutlery adds up. Instead, bring your own reusable bento box to your favorite lunch spot and ask them to make your lunch in it. Simply rewash it and bring it around the next day — just don’t forget your own reusable cutlery, too. This portable bamboo cutlery set even comes with a reusable metal straw and cleaner.
He continued, “Then we’ll run trucks through slush pits and see if they can survive. Run it through, let it freeze sitting overnight. Ice melts, go back into the pool. Freeze the underbody again. Then we’ll run trucks nose-to-tail all in a row so they ingest snow into every orifice to assure the engine can still breathe. You have to be able to drive in a blizzard.”
Yo-Yo test shouldn’t be sole selection criterion, says Sachin Tendulkar | cricket | Waterproof Cabinet Related Video:
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